[quote]Product Name]CAS No. 32780-32-8
Molecular Formula C50H68N14O10
Molecular Weight 1025.2
Purity (HPLC) 98.0%min.
Appearance White powder
Single Impurity (HPLC) 1.0%max
Amino Acid Composition ~10% of theoretical
Peptide Content (N%) ? 80.0%
Water Content(Karl Fischer) ? 6.0%
Acetate Content (HPIC) ? 15.0%
MS(ESI) Consistent
Mass Balance 95.0~105.0%
Sequence AC-Nle-cyclo[Asp-His-D-Phe-Arg-Trp-Lys]-OH
Purity (HPLC) Over than 98.0%
Molecular Formula C50H68N14O10
Molecular Weight 1025.2
Appearance White or off-white powder
IR spectrum in accordance
Note]
Purity (HPLC) 98.0%min. This refers to the % of correct peptide in relation to the net peptide content (i.e. peptide without the counterion). In other words, up to 2% of the peptide weight may be impurities (e.g. deletion/truncation sequences, short peptides). This is measured via HPLC, by detecting the elements besides the main peak that absorb UV at 210-220nm (peptide bond wavelength).
Single Impurity (HPLC) 1.0%max Pretty self-explanatory; max % (of total peptide comp.) that is another single peptide sequence. A lower number is desirable here because a large amount of another single compound may have its own effect, while a "distributed" impurity profile will present fewer compounds in amounts that are analytically/biologically significant.
Amino Acid Composition ~10% of theoretical This should say "±10% of theoretical." This refers to mass spec "fingerprinting" to compare the weight of the compound to its theoretical molecular weight (used in conjunction with HPLC, which identifies purity).
Peptide Content (N%) ? 80.0% Also called net peptide content. This is the % of the total lyophilisate weight (aka 'total/gross' peptide content) that is composed of peptides, both target sequence and peptide impurities.
Water Content(Karl Fischer) ? 6.0% Lyophilization isn't a perfect process; this is the range of total peptide weight that may be residual water.
Acetate Content (HPIC) ? 15.0% The total amount of counterion (acetate). A peptide molecule takes one or two counterions, which would probably be about 5-10% of the weight. Beyond this, a certain amount of unbound counterion will exist as contamination.* Acetate is a compound usually added to things to make them last longer in the body.
Keep in mind that the numbers listed in this COA are ranges and limits. They aren't telling you how much peptide impurity or water is present, only that the content conforms to these less than/greater than statements are true. There could be 0 water and absolutely no impurities (though it's realistically impossible). Some better COAs will list the actual value, but that requires more resources to determine.
Notice ]-OH this is how the peptide molecule is arranged in order, kinda like me saying what you as an individual look like by describing you as : head-neck-torso-leg-leg-arm-arm
Sterile Filtered White lyophilized (freeze-dried) Melanotan II powder. Any peptide can be light and fluffy, compact, dense tiny rocks or a small dense speck or a barely visible spray. There are several different reasons this can occur. Equal amounts of peptide by weight are deposited in a vial via machine. The vial is partially stoppered and transported inside the lyophilizer. The contents of the vial are exposed to the nitrogen flush and air turbulence is created. The peptides may settle in different patterns. Vials are stoppered fully in the lyophilizer. The components of the freezing cycle can vary from vial to vial very easily. The rate and manner of a freeze cycle can effect the physical form of the substance being frozen. For example if the freezing is slightly slower it can lead to formation of large ice crystals and tight very dense compaction of the peptide. Adhesion to the sides of vials can happen when water vapor escapes during a process where it goes from a solid to a gas without ever going through a liquid phase.
Melanotan II peptide temperature, the condenser temperature, chamber pressure, condenser pressure all may vary minutely between vials and account for the visual difference of the freeze-dried product between vials.All of these cycle parameters, the shelf freeze temperature, the product freeze temperature, the freezing "soak" time, the primary drying shelf temperature, cabinet pressure, product temperature for establishment of fill vacuum, secondary shelf drying temperature, and secondary drying time all may contribute to visually different appearance of vial contents if they very even slightly between vials.
Reply With Quote